Duplication of single stranded DNA catalyzed by calf thymus DNA polymerase
نویسندگان
چکیده
منابع مشابه
Single-stranded DNA structure and DNA polymerase activity in the presence of nucleic acid helix-unwinding proteins from calf thymus.
In the preceding articles we have described the isolation and some of the properties of two calf thymus proteins which bind selectively to single-stranded DNA and which appear analogous to previously isolated prokaryotic DNA-unwinding proteins. In the present work we demonstrate two further points of analogy. First, both the calf UP1 and the high salt eluting proteins form protein-rich complexe...
متن کاملApparent stimulation of calf thymus DNA polymerase alpha by ATP.
The mechanism by which millimolar concentrations of ATP stimulate the activity and increase the processivity of calf thymus DNA polymerase alpha has been investigated with poly(dA)/oligo(dT) as template/primer to eliminate possible effects due to primer synthesis. The effect of ATP on the rate of DNA synthesis with this template/primer was found to be dependent upon whether or not the ATP was n...
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A new gene (POLL), has been identified encoding the novel DNA polymerase lambda and mapped to mouse chromosome 19 and at human chromosome 10. DNA polymerase lambda contains all the critical residues involved in DNA binding, nucleotide binding, nucleotide selection, and catalysis of DNA polymerization and has been assigned to family X based on sequence homology with polymerase beta, lambda, mu, ...
متن کاملStudies on polynucleotides, XC. DNA polymerase-catalyzed repair of short DNA duplexes with single-stranded ends.
Short chemically synthesized deoxyribopolynucleotides containing appropriate 5'-phosphate and 3'-hydroxyl end groups can be enzymatically joined end to end, when they are properly aligned, to form bihelical complexes.l1 2 In this way, several short DNA's (Fig. 1, DNA-III-DNA-VI) corresponding to a part of the gene for yeast ala-tRNA have been prepared.2 The products (Fig. 1) are not perfect dup...
متن کاملFurther studies on partially purified calf thymus DNA polymerase a.
Attempts to prevent the urea conversion of a 200-230,000 molecular weight DNA polymerase alpha to a 150-170,000 molecular weight form by the inclusion of protease inhibitors have not been successful. No other method has been found capable of dissociating a 50-70,000 fragment or subunit from the DNA polymerase subunit. Addition of this 50-70,000 subunit to the polymerase subunit does not aid the...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1976
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/3.1.129